plusonePLUS NEW JOURNALCoVolume1 Issue3MultidisciplinaryEnglishJanuary,2023NNY20230424Case Report Optimization of Graptophyllum pictum leaves extraction using a simplex centroid design focused on extracting flavonoids with antioxidant activityEnglishY00anushkaEnglishNGraptophyllum pictum (L.) Griff, which is called “daun ungu” in Indonesia, is a medicinal plant that contained flavonoids with antioxidant activity. The different extractor solvents (water, acetone, methanol, and ethanol) and their combinations were studied using a simplex centroid design through the Design Expert 13.0 program. From the optimized crude extract, it was investigated for total flavonoid content (TFC) using the colorimetric method (AlCl3), as well as the antioxidant activity using 2,2-diphenyl-1-picryl hydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. The optimum condition was obtained at a desirability value of 0.745 with a water solvent system at a proportion of 1.00 (100%) which resulted in a TFC of 10.464 mg QE/g DW, the antioxidant activity of DPPH 2.179 μmol TE/g DW, and FRAPf 22.009 μmol TE/g DW. The method was verified and analyzed based on the percentage of residual standard error, which resulted inEnglishsimplex, methodhttps://plusone.ubipayroll.com/abstract?id=5References1619Abenavoli L, Larussa T, Corea A, Procopio AC, Boccuto L, Dallio M, Federico A, Luzza F. Dietary polyphenols and non-alcoholic fatty liver disease. Nutrients, 2021; 13:494; doi:10.3390/nu13020494.and;nbsp;https://doi.org/10.3390/nu13020494Alara OR, Abdurahman NH, Ukaegbu CI, Azhari NH. Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique. Ind Crops Prod, 2018; 122:533-44; doi:10.1016/j.indcrop.2018.06.034.and;nbsp;https://doi.org/10.1016/j.indcrop.2018.06.034Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem, 1996; 239(1):70-6; doi:10.1006/abio.1996.0292.and;nbsp;https://doi.org/10.1006/abio.1996.0292Blois MS. Antioxidant determinations by the use of a stable free radical. Nature, 1958; 181:1199-200; doi:10.1038/1811199a0and;nbsp;https://doi.org/10.1038/1811199a0Carey CC, Lucey A, Doyle L. Flavonoid containing polyphenol consumption and recovery from exercise-induced muscle damage: a systematic review and meta-analysis. Sport Med, 2021; 51:1293-316; doi:10.1007/s40279-021-01440-x.and;nbsp;https://doi.org/10.1007/s40279-021-01440-xDos Santos C, Mizobucchi AL, Escaramboni B, Lopes BP, Angolini CFF, Eberlin MN, De Toledo KA, Nand;uacute;and;ntilde;ez EG. Optimization of Eugenia punicifolia (Kunth) D. C. leaf extraction using a simplex centroid design focused on extracting phenolics with antioxidant and antiproliferative activities. BMC Chem, 2020; 14:34; doi:10.1186/s13065-020-00686-2.and;nbsp;https://doi.org/10.1186/s13065-020-00686-2